Figure 1.

Proper association and dissociation of NHEJ factors depend on RBM14. (A) RBM14 is required for recruitment of XRCC4 and XLF to damaged chromatin. U2OS cells were treated with 500 µM phleomycin for the indicated times. Cells were fractionated in lysis buffer containing RNaseA (chromatin; left) or lysed as whole cell extracts (right). (B) RBM14 knockdown inhibits release of KU proteins from damaged chromatin. U2OS cells were treated with 500 µM phleomycin for 60 min, and then left to recover following phleomycin removal. Cells were collected at the indicated time points. KD: knockdown.