Author response image 4. Amplitude of bioluminescence signals originating from motor neurons does not increase with the intensity of voltage applied to induce acoustic stimuli.
(A) As shown here for 10 fish tested in control conditions and with pancuronium bromide, larger voltages actually led to decreased amplitude of the bioluminescence signals before paralysis (mean bioluminescence amplitudes across fish = 28.3 +/- 1.7; 25.7 +/- 4.6; 14.9 +/- 4.7 photons /10ms for stimuli voltage of respectively 1, 2 or 4 V, p = 0.03) and no correlation after paralysis. Furthermore, one can note that the stimulus voltage used in paralyzed conditions was equal or higher than in control conditions. (B) As shown here for 10 control fish and 10 immotile mutants, larger voltages did not lead to larger amplitude of the bioluminescence signals: there was no correlation in control siblings while immotile mutants showed smaller signals for larger stimuli (mean bioluminescence amplitudes across fish = 13.9 +/- 3.6; 10.5 +/- 6.4; 7.9 +/- 7.3; 5.0 =:- 2.0 photons /10ms for respective stimuli voltage of 2, 3, 5 or 8 V; p = 0.001). Furthermore, one can note that the stimulus voltage used in immotile mutants (mean = 3.9 +/- 0.1 V) was higher than in control conditions (mean = 2.4 +/- 0.01 V, p < 0.001).