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. 2017 Jul 6;15(7):e2001246. doi: 10.1371/journal.pbio.2001246

Fig 1. Isoflurane exposure results in overgrowth of dendritic arbors.

Fig 1

(A) A schematic diagram of isoflurane exposure procedure for morphology examination. (B) Sample confocal image of dentate gyrus granule cell (DGCs) infected with retrovirus expressing green florescent protein (GFP) (scale bar: 100 μm). Representative confocal images (C) and tracings (D) of individual control and isoflurane-exposed GFP+ neurons at postnatal day (P) 30 exhibiting overgrowth in the isoflurane group relative to control conditions (scale bar: 10 μm for both C and D). Summaries of total dendritic length (E) and Sholl analysis of dendritic complexity (F) of GFP+ neurons show marked overgrowth of dendritic arbors. Numbers associated with bar graph indicate the number of neurons examined from at least 5 animals per group. The same groups of neurons were examined in (E) and (F). Values represent mean ± SEM (**p < 0.01; Student t test for E and *p < 0.0001 ANOVA for F). Underlying data in S1 Data under Fig 1F tab.