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The acidic patch residue H2A E57 is important for binding FACT. (A) Co-IP analysis using anti-myc beads of nuclease-treated whole-cell extracts containing Spt16-myc and HA-tagged WT or indicated mutant histones. Untagged WT histones are coexpressed. (B) Quantification of the percent IP of the tagged band relative to the untagged band. Graphs represent the mean and SEM of at least three independent replicates. ** P < 0.01.
