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(A) Schematic of the germarium. The germarium is divided into four regions (1, 2a, 2b, and 3), and contains two FSCs at the region 2a/2b border. (B) Representative images of a mosaic germarium with one FSC marked by the lack of GFP (“single FSC clone”) and a fully marked germarium with both FSCs marked by the lack of GFP (“double FSC clone”). (C) Results from the pilot screen assayed at 3, 6, 12, 16, and 20 days ACI. arm, hop, and sec16 have hypo-competition phenotypes.
