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. 2017 May 16;206(3):1417–1428. doi: 10.1534/genetics.117.201202

Figure 3.

Figure 3

(A-B) Wildtype germaria stained for Sec16 (green) and FasIII (red). Sec16 localizes to discrete puncta throughout all cells in the germarium. (A′-D′) Sec16 channel only. (B) Overexpression of Sec31-Cherry (red) in follicle cells with 10930-Gal4 at 7 days post eclosion. Sec16 (green) colocalizes with Sec31 within the FasIII-positive (blue) follicle cells, indicating that it is at ERESs. Boxed region magnified in inset to show co-localization. (C-D) Germaria with UAS-sec16 RNAi and tub-Gal80ts that lack a Gal4 driver as a control or have a 10930-Gal4 stained for Sec16 (green) and FasIII (red) 4 days after shift to 29°. Sec16 puncta are visible in the control (C) but are absent specifically in the follicle cells (as indicated by FasIII, red) of germaria expressing Sec16 RNAi (D). Boxed regions magnified in insets to show loss of Sec16 puncta in upon RNAi knockdown. (E) Germarium with a GFP− sec16A FSC clone stained for GFP (the clonal marker, green), FasIII (red), and Sec16 (white). Puncta of Sec16 are clearly visible in sec16A (GFP−) follicle cells. White box outlines a region with GFP− follicle cells and is magnified in E′′′. (E′) FasIII and GFP channels, (E′′) Sec 16 channel. White scale bar is approximately 10 μm.