Figure 5.

Germaria with Shi^A (A-B), Wus^A (C-D), Crag^A (E-F), or Sec16^A (G-H) clones stained for GFP (clonal marker, green), DE-cad (red), and DAPI (blue). DE-cad channel shown separately in A′-H′. Images of clones in the germarium shown in A, C, E, and G; images of clones in budded follicles shown in B, D, F, and H. The positions of mutant follicle cells are indicated by solid yellow lines. Boxed regions in A-H are magnified in A′-H′. Yellow arrows indicate GFP⁻ homozygous mutant follicle cells. DE-cad is present on the apical membranes of heterozygous, GFP⁺ follicle cells in all cases but absent from the apical domains of Shi^A follicle cells in the germarium and in budded follicles; absent from the apical domains of Wus^A follicle cells in the germarium and disrupted in Wus^A follicle cells of budded follicles; present but mildly disrupted in Crag^A and Sec16^A follicle cells in the germarium; and normally localized in Crag^A and Sec16^A follicle cells in budded follicles.