Figure 6.

(A–D) Germaria with (A) Sec16^A, (B) Shi^A, (C) Wus^A, or (D) Crag^A clones stained for pERK (red), GFP (clonal marker, green), and DAPI (blue). Images are oriented so that the GFP⁻ FSC is at the top of the image as shown in (A–D), and the GFP⁺ FSC is at the bottom as shown in (A′–D′). Each FSC (indicated by a yellow arrow within the white boxes) is shown in the insets to the right of each image. The insets show a merge of all three channels, and the pERK channel alone. Each image is an optical section selected from a z-stack to best capture the indicated FSC. For all four genotypes, the homozygous mutant GFP⁻ FSC has low or undetectable pERK staining, and the heterozygous GFP⁺ FSC in the same germarium has normal levels of pERK staining. (E) Quantification of the frequency of pERK staining in FSCs that are heterozygous (GFP⁺, gray boxes) or homozygous (GFP⁻, white boxes) for the chromosome arm with the allele indicated on the x-axis. Three to five independent trials were conducted for each genotype; each ● indicates the mean values from a single trial. N = 53–77 germaria scored (total from all three to five trials) for each genotype. Boxes show the mean and the first and third quartiles of the means from each trial. * P < 0.01.