Figure 9.

Increased apoptosis in the brain of adult neuron-specific Psn and Nct cKD flies. (A) Apoptotic cells (marked by arrowheads) were identified by TUNEL staining. Bar, 10 μm. (B) Quantification of TUNEL+ cells. The number of TUNEL+ cells is increased in AN-Psn cKD, AN-Nct cKD, and AN-Psn/Nct cKD brains compared to control brains; n = 11–14 brains per genotype at 30 days of age. All data are expressed as mean ± SEM. Statistical analysis was performed using one-way ANOVA with Dunnett’s post hoc comparisons. ****P < 0.0001.