Figure 3.

Interaction between RIG-3 and CAM-1. (A) YFP reconstitution across the dorsal cord in the background of Pacr-2::mCherry::RAB-3 that shows expression along both the cords: RIG-3 expressed under the unc-129 promoter interacts with CAM-1 expressed in body-wall muscle (myo-3 promoter) along the dorsal cord, but not along the ventral cord (top rows; arrowheads). The lower left set of images show the control, where RIG-3 does not interact with CAM-1(ΔIg). RIG-3(TMD) also shows interaction with CAM-1 along the dorsal cord (lower right panels, arrowhead). (B) Coimmunoprecipitation experiment: the panel shows the coimmunoprecipitation (Co-IP) blot. Protein extracts isolated from WT and CAM-1::GFP animals were allowed to interact with GFP antibody and pulled down using ProteinA/G beads. As a control, the same protein extracts were allowed to bind to beads but without GFP antibody. The coimmunoprecipitated complex was probed with the anti-RIG-3 antibody. The RIG-3 containing complex was pulled down only in the lane containing the CAM-1::GFP animals, and not in any of the other three control lanes (arrowhead). Co-IP analysis indicates interaction between CAM-1 and RIG-3.