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. Author manuscript; available in PMC: 2017 Jul 6.
Published in final edited form as: Acta Biomater. 2016 Jan 21;33:264–274. doi: 10.1016/j.actbio.2016.01.026

Figure 4.

Figure 4

Magnetic induction heating (MIH) with Fe3O4 nanoparticles in an AC magnetic field improves the viability of hUCM-MSCs post-cryopreservation by vitrification: (a) Representative fluorescence micrographs showing live (green) and dead (read) staining of hUCM-MSCs post-cryopreservation by vitrification with and without MIH during rewarming; (b) Quantitative data of the viability of hUCM-MSCs post-cryopreservation by vitrification with and without MIH during rewarming. (c) Typical two-channel flow cytometry data (Annexin V-FITC and PI double staining), (d) quantitative data of different cell populations from the flow cytometry analysis, (e) cell viability calculated as the percentage of cells with negative staining of both Annexin V and PI, and (f) Attachment efficiency of the hUCM-MSCs after cryopreservation by vitrification with MIH (0.05% (w/v) Fe3O4 nanoparticles) at the different currents during rewarming; (g) Proliferation of fresh hUCM-MSCs and the cells cryopreserved by vitrification with MIH (15 A and 0.05% (w/v) Fe3O4 nanoparticles) during rewarming. *, p <0.05; **, p <0.01.