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Effects of Fe3O4 nanoparticles and cryoprotectant solution in the straw on the vitrified cryopreservation.
| Trehalose | Fe3O4 NPs | PROH:EG (1:1) | ||
|---|---|---|---|---|
| 1.0 M | 1.5 M | 2.0 M | ||
| W/O | W/O | − | − | + |
| W | − | − | − | |
| 0.5 M | W/O | − | − | + |
| W | − | − | + | |
| 1.0 M | W/O | − | −/+ | + |
| W | − | − | + | |
The symbols + and − represent apparent vitrification and appearance of visible ice crystal (opacity) in the whole solution volume, respectively. The symbol “−/+” indicates partial apparent vitrification.
