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(A) Immunofluorescence for topo IIα on HTETOP metaphase cells grown in the absence (topo IIαON) and presence (topo IIαOFF) of doxycycline for 48 hours. (B) Immunofluorescence for the SMC2 subunit of condensin on HTETOP metaphase spreads isolated from cells grown in the presence and absence of doxycycline for 72 hours. The normal axial distribution of SMC2 is detected following topo IIα depletion. Scale bar, 10 μm.
