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. Author manuscript; available in PMC: 2017 Jul 6.
Published in final edited form as: J Cell Sci. 2007 Oct 23;120(Pt 22):3952–3964. doi: 10.1242/jcs.013730

Fig. 5.

Fig. 5

Immunofluorescence of topo IIα-expressing and topo IIα-depleted (72 hours dox) HTETOP cells. (A) Metaphases (cytospun, no colcemid) stained using an antibody against CENP-A (green); (B) partial chromosome spreads (colcemid) stained for CENP-F (green); (C) partial metaphase spreads (colcemid) stained for CENP-E (green)); (D) typical metaphase cells (grown in situ, no colcemid) stained for CENP-C (green) and Aurora B (red); (E) immuno-FISH of partial chromosome spreads (colcemid) showing the typical banding pattern generated using an antibody raised against acetylated histone H3 (Lysines 1-21) (green). The centromeric regions have been identified using a pan-alphoid DNA probe (red). The white arrowheads highlight examples of hypoacetylated centromeric and pericentromeric domains. DNA/DAPI - blue. Scale bars, 10 μm.

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