Skip to main content
. Author manuscript; available in PMC: 2017 Jul 6.
Published in final edited form as: J Cell Sci. 2007 Oct 23;120(Pt 22):3952–3964. doi: 10.1242/jcs.013730

Fig. 6.

Fig. 6

The effect of topo IIα depletion on anaphase PICH-coated threads. HTETOP cells expressing topo IIα (A), or depleted of topo IIα (48 hours dox) (B) were fixed and permeabilised before being co-stained for PICH (green) and CENP-B (red). Shown are cells in anaphase, or undergoing aberrant cytokinesis. Arrowheads indicate examples of PICH threads that are not associated with DAPI-stained chromatin bridges. Scale bar, 10 μm. (C) Quantification of the number of PICH threads for anaphase cells expressing topo IIα (topo IIαON) and for cells treated for 48 hours with doxycycline (topo IIαOFF). Fifty anaphase cells were analysed for each and the stage was determined by the distance between segregating sister kinetochores.