Figure 2.
Thermostability of MelBSt solubilized in a MGA (MGA-C10, MGA-C11, MGA-C12, MGA-C13, MGA-C14, or MGA-C15) or DDM. The protein was extracted from E. coli membranes by treatment with 1.5 wt% individual detergent at four different temperatures (0, 45, 55, and 65°C) for 90 min. The solubilized MelBSt was separated by ultracentrifugation and visualized by Western blot (a). The amounts of the soluble MelBSt were expressed as percentages of total MelBSt in the untreated membrane (Memb) and presented in histograms (b). Error bars, SEM, n=3.