Fig. 2.

MDM2 depletion by fulvestrant does not correlate with p53 expression or activation. MCF7 cells (wild-type for p53) were cultured in the presence of different concentrations of estradiol (E2) for 72 h, with or without fulvestrant (Fulv). The p53 (A) and p21 (B) protein levels were measured by Western blot and normalized to the levels of β-actin. No significant changes were observed. (C) Representative Western blots from three independent experiments are shown.