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. Author manuscript; available in PMC: 2017 Jul 6.
Published in final edited form as: Cancer Lett. 2014 Apr 18;350(1-2):52–60. doi: 10.1016/j.canlet.2014.04.009

Fig. 6.

Fig. 6

Combination of fulvestrant and cytotoxic drugs induces altered cell cycle distribution, apoptosis, and senescence. MCF7 and T47D cells were treated for 72 h with fulvestrant (Fulv), doxorubicin (Dox), paclitaxel (Tax), and etoposide (VP16) either alone or in combination. A representative histogram (A) and bar graph depiction (B) of MCF7 cell cycle distribution are shown for fulvestrant, etoposide, or the combination of fulvestrant and etoposide. Quantitation of cell cycle distribution for all treatments is shown in Table 2. (C) Levels of cleaved PARP and cleaved caspase 8 in MCF7 cells were analyzed by Western blot and normalized to full length PARP and caspase 8, respectively. Representative Western blots of MCF7 lysates from three independent experiments are shown below the corresponding graph. (D) Senescence in MCF7 treated cells was evaluated based on β-galactosidase staining. *P < 0.05 compared to vehicle control treatment. **P < 0.05 for combination treatments compared to each agent alone or to control.