Table 2. Comparison of reference compounds IC₅₀ (uM) values generated from single and multiple concentrations methods and literature reports.

Compounds	Single Dose Closed State	Six Dose Closed State	Literature Closed State	Single Dose Inactivated state	Six Dose Inactivated state	Literature Inactivated state	Reference
Tetrodotoxin	0.017	0.016	0.02	0.009	0.011	0.02~0.03	[20],[19]
Amitriptyline	3.74	3.18	10.01	0.147	0.245	0.56~1	[16],[19]
Carbamazepine	497.4	441	>100	88.4	120	29~101	[19],[20]
Flecainide	6.69	8.6	>10	2.69	5.1	0.85	[16]
Lamotrigine	311.9	243	>100	16.5	27.4	34~79	[19],[20]
Mexiletine	117.7	85	121.94	15.3	16.4	30.36~47	[19]
Tetracaine	20.7	27.0	20~30	0.264	0.245	0.1~0.43	[16],[19],[20]
CNV1014802	46.7	12.3	54	1.41	1.4	6.3	[21]
GX-936	0.007	0.0051		0.003	0.0043