Fig 1. Time-course analysis of DENV and CHIKV (co)- infection in hPBMCs cell-free supernatants from PBMCs of three different donors infected with DENV or CHIKV or co-infected with both viruses were collected at 12, 24, 48 and 72hpi and analysed by qPCR to determine the number of specific viral particles expressed as genome equivalent copies per mL (GEc/mL).

(A) Time-course analysis of DENV production during mono-infection at MOI of 1 and during the co-infection with CHIKV at different MOIs. (B) Time-course analysis of CHIKV production during mono-infection at MOI of 1 and during the co-infection with DENV at different MOIs. (C) Effect of co-infection on CHIKV production in hPBMCs, of DENV MOI 1 during co-infection with CHIKV or UV-CHIKV relative to mono-infection. (D) Effect of the co-infection with replicative and non-replicative DENV on CHIKV production in hPBMCs, For C and D, bars represent an average % of infection normalized to relative mono-infections of three donor’s ± SEM.