Fig 3. Differential role of type I interferons in CHIKV and DENV infection.

(A) Concentration of IFN-α, IFN-β and IFN-ω in the supernatants of mono- and co-infected cells at 24 hpi. (B) Concentration of IFN-α, IFN-β and IFN-ω in the supernatants of mono- and CHIKV/UV-DENV co-infected cells at 24 hpi. (C/D) Effect of IFN-β and IFN-ω on CHIKV and DENV replication. Vero-WHO cells were pre-treated with IFN-β or IFN-ω for 24 h and infected with CHIKV (C) or DENV (D) at MOI of 1. The number of genome equivalent copies per mL (GEc/mL) in cell-free supernatant harvested at 24 hpi was determined by RT-qPCR. Results are represented as mean ± SEM of three independent experiments.