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. 2017 Jun 22;60(6):181–188. doi: 10.3345/kjp.2017.60.6.181

Fig. 4. Activity of cleaved caspase-3 in the neuronal cells and astrocytes. Caspase-3 was assayed using a caspase-3/CPP32 colorimetric assay kit in the neuronal cells (A) and astrocytes (B). Neuronal cells and astrocytes were plated 24 hours before the induction of apoptosis. The cells were pretreated with EPO for 48 hours before a hypoxic insult at 0.1 U/mL in the neuronal cells, at 10 U/mL in the astrocytes. H, hypoxic group; H+erythropoietin (EPO), hypoxic group pretreated with EPO; N, normoxic group. *P<0.05, statistically significant vs. N group. #P<0.05, statistically significant vs. H group.

Fig. 4