Fig. 5. (A) Western blot results of mitogen-activated protein kinase (MAPK) pathway in the neuronal cells. Expression of antibodies against phosphorylated extracellular signal-regulated kinase (pho-ERK1/2), p38MAPK (pho-p38MAPK), and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK; pho-SAPK/JNK) was analyzed. (B-D) Expression of ERK1/2, p38 MAPK, SAPK/JNK was used as a loading control. Quantification of Western blot to determine the relative phosphorylation ratio of the MAPK pathway signals in the neuronal cells. The relative phosphorylation ratio of ERK-1/-2 was significantly decreased in H+erythropoietin (EPO) at 15 hours after hypoxia. H, hypoxic group; H+EPO, hypoxic group pretreated with EPO; N, normoxic group. *P<0.05, statistically significant vs. N group. #P<0.05, statistically significant vs. H group.
