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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Mol Cancer Res. 2017 Mar 30;15(7):831–841. doi: 10.1158/1541-7786.MCR-16-0218

Figure 1. Detection of DNA damage and APE1 mRNA levels induced by AOM.

Figure 1

Total DNA from colonic crypts was isolated 24, 48, and 72 hrs after AOM treatment from WT and Apex1+/- mice and analyzed for mtDNA damage, mtDNA abundance, and nDNA damage by QPCR. (A) Frequency of mtDNA lesions in WT mice. (B) Frequency of mtDNA lesions in Apex1+/- mice. C) Relative mtDNA abundance in WT. (D) Relative mtDNA abundance in Apex1+/- mice. (E) Frequency of nDNA lesions in WT mice. (F) Frequency of nDNA lesions in Apex1+/- mice. Results are expressed as mean ± SEM values for 3 QPCRs performed in triplicate. (G) Relative APE1 mRNA levels in WT mice 48 hours after AOM treatment. (H) Relative APE1 mRNA levels in Apex1+/- mice 48 hours after AOM treatment. WT and Apex1+/- mice: N=8 for Saline treated mice; N=9 mice 24 hr AOM treatment; N=9 mice 48 hr AOM treatment; N=8 mice, 72 hr AOM treatment. Asterisks (*) denote statistical significance (One-way ANOVA, *P<0.05, **P<0.01).