. Author manuscript; available in PMC: 2018 Jul 1.

Published in final edited form as: Mol Cancer Res. 2017 Mar 30;15(7):831–841. doi: 10.1158/1541-7786.MCR-16-0218

Table 2.

Histopathological analysis of AOM-induced tumors in WT and Apex1^+/- mice.

			Parameters
Groups	Nuclear/cytoplasmic ratio	Epithelial stratification	Nuclear dispolarity	Goblet depletion	Structural abnormality
WT control tissue	0±0.10	0±0.13	0±0.16	0.17±0.10	0±0.10
WT tumor	1.8±0.10^a	1.6±0.13^a,^b	1.6±0.16^a	2.0±0.10^a	1.8±0.10^a
Apex1^+/- control tissue	0±0	0±0	0±0	0±0	0±0
Apex1^+/- tumor	2.0±0.09^a	2.0±0.12 ^a	1.8±0.16^a	2.0±0.09^a	2.0±0.09^a

The grade of histological abnormality was scored using the following five parameters: a) nuclear/cytoplasmic ratio(<25%:0, 25-50%:1, >50%:2; b) epithelial stratification (none: 0,mild: 1,severe: 2); c) nuclear dispolarity (none: 0, mild: 1, severe: 2); d) goblet depletion (null to mild: 0, moderate: 1, severe: 2); e) structural abnormality (none: 0, mild: 1,severe: 2). Values represented mean ± SEM. One-way ANOVA,

P<0.05 compared to WT control tissue group;

P<0.05 compared to Apex1^+/- tumor group.