Figure 2.

CYP2E1 protein content and activity, in vitro CCl₄ exposure. Control mice given an olive oil injection and euthanized 72 h later were evaluated for (A) Cyp2e1 mRNA and (B, C) CYP2E1 protein from whole liver. (D) Cyp2e1 expression was determined in isolated hepatocytes from mice that did not receive olive oil injection. (E) CYP2E1 activity in microsomes isolated from whole liver of mice that received olive oil injection was determined by the hydroxylation of p-nitrophenol to p-nitrocatechol. (F, G) Primary hepatocytes were isolated from 6N and 6J mice and exposed to 0, 1, 5, and 10 mM CCl₄ for 24 h. (F) Death ratio was calculated from the amount of lactate dehydrogenase (LDH) released in the media versus the total amount of LDH in the cells and media. (G) Cell morphology was evaluated in micrographs taken at the end of the 24-h incubation.