Figure 4.

Hepatic neutrophil accumulation after CCl₄ exposure. Mice were exposed to CCl₄ and euthanized 12, 24, 48, 72, or 96 h later. (A) Cxcl2 and (B) Cxcr2 transcripts were quantified in whole-liver cDNA samples using real-time polymerase chain reaction (PCR). (C) Quantification of chloracetate esterase (CAE) staining used to quantify the number of neutrophils in the liver at baseline (oil) and 12 and 24 h after CCl₄. (D) Representative images of CAE⁺ cells with neutrophil morphology (pink staining). Black dotted line is outlining CAE⁺ cells and red solid line is are of image enlarged in far right panel. The + indicates central veins in the images. n = 5–6 per group. *p ≤ 0.05 when comparing substrains at a single time point; +p ≤ 0.05 when comparing the indicated CCl₄ time point to the oil (control) of the same substrain.