Figure 3.

Expression of AP-1 members in CaCxSLCs. (A) Transcript levels of AP-1 family members. Representative cropped gel photographs showing relative transcript levels of indicated AP-1 members in cDNA prepared from CaCxSLCs and non-CaCxSLCs by qRT-PCR. cDNA from parental SiHa cells were used as reference. GAPDH qRT-PCR was used as input control for normalization as described in Methods (i). The gels were run under the same experimental conditions. Normalized fold change in transcript levels is expressed as mean ± SD of three independent experiments (ii). *p-value < 0.05 vs. parental SiHa cells. (B) Representative cropped blots showing expression levels of c-Jun, c-Fos, JunB, JunD and Fra-1 in total cellular extracts derived from CaCxSLCs culture and non-CaCxSLCs or presorted SiHa cultures. β-actin was used as input control (i). The abundance ratio of each AP-1 members to β-actin was analyzed by densitometry. The gels were run under the same experimental conditions. The relative normalized fold change in the protein is expressed as the mean ± SD of three independent experiments (ii). *p-value < 0.05 vs. parental SiHa cells. (C) Intracellular expression of cellular AP-1 protein in CaCxSLCs. Representative confocal immunofluuorescence image of AP-1 family members in day10 cultured cervicosphere CaCxSLCs and non-CaCxSLCs cultures. Parental SiHa cells were used as reference.