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. 2017 Jul 6;7:4792. doi: 10.1038/s41598-017-05029-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dasatinib inhibits cell proliferation in MRMT1-luc2 cells in a dose-dependent manner in vitro. MRMT1-luc2 cells were treated with increasing concentrations of dasitanib for 48 h and assayed by the (a) MTT assay, (b) the BrdU assay, and (c) the RealTime-Glo™ assay. IC50, the concentration needed to inhibit viability or proliferation by 50. Data were normalized to cells treated with 0.1% DMSO (negative controls, 100% activity) and blank controls, e.g. wells without cells (0% activity) and expressed as percentage of these controls. Data are displayed as mean ± SEM. Normalized response is compared to common log of dasatinib concentration (µM) and the IC50 calculated using a variable slope. Data from the MTT assay represents one of three independent experiments done in quadruplicates. Data from the BrDU and RealTime-Glo™ assays represents one experiment done in triplicates to confirm the results from the MTT assay.