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. 2017 Jul 6;7:4803. doi: 10.1038/s41598-017-05073-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of the binding sites of of GdmRIII. (a) DNase I footprinting assay of the elaF promoter protected by GdmRIII. The middle electropherogram represents the sequencing reaction without GdmRIII protein and the lower electropherogram represents the reaction with GdmRIII protein added. The regions where the blue peaks are constantly lower than the red peaks are considered to be protected by GdmRIII; (b) the nucleotide sequence of the elaF promoter region and the GdmRIII binding sites. The numbers indicate the distance (nt) from the translational start point. The solid lines indicate the GdmRIII binding sites. (c) the conserved sequence among GdmRIII binding sites.