Figure 3.

Participation of polysulfide in the 1,4-NQ-mediated phosphorylation of Akt and CREB in primary mouse hepatocytes. (A) Cells were exposed to the indicated concentrations of 1,4-NQ for 30 min in the presence of Na₂S₄ at 0, 10 (A), or 100 (B) µM. Akt and CREB phosphorylation was determined by western blotting (right). Representative blots are shown from three independent experiments. Band intensities were normalized to those of total Akt and CREB, respectively (left). Intensities are presented as fold induced relative to results with 0 µM 1,4-NQ exposure. Each value is the mean ± standard error for three independent experiments. *P < 0.05 and **P < 0.01, compared with 0 µM 1,4-NQ exposure.