Table 3.
Invasion of pathogens into MAC-T cells.
| Pathogen | strain | Attributed phenotype | Co-culture time | Na | |
|---|---|---|---|---|---|
| 20 min | 60 min | ||||
| S. aureus | 1027 | clinicalb | 3.0 ± 1.4 | 12.3 ± 6.6 | 4 |
| RF122 | clinicalb | 0.7 ± 0.4 | 13.0 ± 0.7 | 2 | |
| B | sub-clinicalb | 0.6 | 3.8 | 1 | |
| D | sub-clinicalb | 6.2 | 13.4 | 1 | |
| E. coli | 1303 | clinicalb | 0.00 | 0.47 ± 0.27 | 2 |
| ECC-Z | persistentc | 0.02 ± 0.003 | 0.045 ± 0.02 | 3 | |
| ECA-0157 | transientc | 0.007 ± 0.001 | 0.02 ± 0.005 | 2 | |
| ECA-727 | transientc | 0.006 ± 0.001 | 0.02 ± 0.004 | 2 | |
Values represent mean percentages (±SEM) of the inoculum found inside the cells after co-culturing pathogen and host cells for the times as indicated. aNumber of independent experiments, each assayed in duplicate. bOriginally isolated from clinical or sub-clinical cases of mastitis, cf ref. 65; cwas shown to cause transient or persistent infection of udder cells, cf ref. 44.