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. 2017 Jul 6;7:4793. doi: 10.1038/s41598-017-04116-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PLC/RF5 HCC cells with HNF-1β overexpression strongly expressed liver progenitor cell markers. (A,B) Detection of HNF-1β expression in HCC cells and HNF-1β-overexpressed HCC cells. OE: HNF-1β overexpression. (C,D) RT-PCR and western blotting were performed to detect the expression of the HPC markers (CK7, CK19, SOX9 and CD133). (*P < 0.05, **P < 0.01) Mean ± S.E.M. (E–H) Immunofluorescence staining was used to identify the expression level of the phenotypes (CK7, CK19, SOX9 and CD133) (scale bar = 100 μm). (I) A colony formation assay was used to detect the difference in the stemness between HCC cells and HNF-1β-overexpressed HCC cells.