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. 2017 Jul 6;7:4793. doi: 10.1038/s41598-017-04116-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HNF-1β overexpression promoted invasion of PLC/RF5 HCC cells. (A,B) Transwell invasion assay was used to observe the invasion ability of the HCCs and the HNF-1β-overexpressed HCC cells. (C,D) RT-PCR and western blotting was performed to detect the expression of the EMT markers (E-cadherin and N-cadherin) in HCC cells and the HNF-1β-overexpressed HCC cells. (*P < 0.05, **P < 0.01) Mean ± S.E.M. (E,F) Immunofluorescence staining was used to identify the expression level of the EMT markers (E-CAD and N-CAD) (scale bar = 100 μm).