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. 2017 Jul 6;7:4793. doi: 10.1038/s41598-017-04116-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HNF-1β upregulated expression of Notch signalling-related genes in PLC/RF5 HCC cells. (A,B) The activation of NOTCH1 and HES1 in HCC cells was detected by RT-PCR and western blotting analysis. (C,D) The inhibition of the Notch pathway by DAPT or shRNA was confirmed using RT-PCR and western blotting. (E,F) RT-PCR and western blotting were used to determine the expression of CK7, CK19, SOX9 and CD133 after treatment with DAPT or Notch1 shRNA in HNF-1β-overexpressed HCC cells. (G,H) The expression of EMT markers was detected by RT-PCR and western blotting. (*P < 0.05, **P < 0.01, *** P < 0.001) Mean ± S.E.M. OE: HNF-1β overexpression. sh-Ctrl: negative control shRNA. sh-Notch1: Notch1 shRNA.