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SLP-76 phosphorylation in the presence of SYK and ITAM-containing receptors. S2-cells were co-transfected with the indicated proteins and GFP as a transfection marker. Equal numbers of FACS-enriched GFP+ S2-cells were lysed and analyzed by western blot for SLP-76 phosphorylation and the expression of the transfected proteins. Expression of NKp80 and CRACC was verified by FACS analysis (not shown). Results are representative of at least five independent experiments.
