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Co-expression of Fyn enhances ZAP70 activity. S2 cells were co-transfected with GFP, SLP-76, CD3ζ, and (A) SYK or (B) ZAP70 in the absence (left lanes) or presence (right lanes) of Fyn. Equal numbers of FACS-enriched GFP+ S2-cells were lysed and analyzed by western blot for SLP-76 and CD3ζ phosphorylation and expression of the transfected proteins. All western blots are representative of at least three independent experiments.
