Figure 1.

Heterotopic transplantation, revascularization, and luminal occlusion of the graft. (A) Small bowel resections are extracted from C57BL/6 mice. (B) For isogeneic transplantation, the resection (arrow) is implanted into subcutaneous tissue in the neck of C57BL/6 mice. (C) The graft is freed from the pouch and harvested from the neck of the recipient 14 days posttransplantation. (D–F) Grafts in the neck of recipient animals observed in situ present a decreased length but are otherwise macroscopically intact. Blood vessels from the surrounding tissue stretch toward the graft where they form a dense network (twofold magnification). (G) Histologic cross sections of freshly isolated small intestine (day 0). Small bowel resections are extracted from C57BL/6 mice, implanted into C57BL/6 mice for isogeneic transplantation, and explanted at (H) day 2, (I) day 6, and (J) day 14 after transplantation. Transmitted light microscopy, H&E staining. Grafts revealed luminal occlusion.