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. 2017 Jul 7;4:96. doi: 10.3389/fmed.2017.00096

Figure 2.

Figure 2

Collagen layer thickness and Tgf-β, Col1a1, and Col3a1 mRNA are significantly increased in grafts from the heterotopic transplantation model in a time-dependent manner. Small bowel resections are extracted from RAG2 knockout mice and implanted into RAG2 knockout mice for isogeneic heterotopic transplantations. (A) Sirius Red staining. Transmission light microscopy and polarized light microscopy. (B) Collagen layer thickness measurement using transmission light microscopy confirmed significantly increased collagen layer thickness in a time-dependent manner (**p < 0.01, ***p < 0.001, ANOVA, Dunn’s multiple comparison test). Thickness was calculated from at least eight places in representative areas at 10-fold magnification for each single graft. (C) Tgf-β qPCR. (D) Col1a1. (E) Col1a3. *p < 0.05, ***p < 0.001, Kruskal–Wallis test, Dunn’s multiple comparison test.