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. 2017 Apr 28;16(7):1377–1392. doi: 10.1074/mcp.M116.058321

Fig. 8.

Fig. 8.

Messenger RNA and protein expression of SQSTM1 and phosphorylated SQSTM1 on serine residue on amino acid position 28 and threonine 269/serine 272 in platinum sensitive M019i and resistant M109iCis cells using qRT-PCR and Western blot analysis. Statistical differences were calculated using Two-Way ANOVA with multiple comparisons using posthoc Tukey test for paired treatment groups of sensitive and resistant cells and using One-Way ANOVA with multiple comparisons using posthoc Holm-Sidak method within treatment groups of resistant cell line. Each bar shows mean ± S.E. of three separate experiments. A, SQSTM1 mRNA expression, (B) Serine 28 phosphorylated SQSTM1, (C) Threonine 269/serine 272 phosphorylated SQSTM1, (D) Serine 28 phosphorylated SQSTM1 to SQSTM1, (E) Threonine 269/serine 272 phosphorylated SQSTM1 to SQSTM1.