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. 2017 May 18;292(27):11423–11430. doi: 10.1074/jbc.M117.790675

Figure 1.

Figure 1.

NleB/SseK orthologs have differential activities toward host NF-κB signaling. A, analysis of IκBα degradation and NF-κB p65 subunit nuclear translocation. HEK 293T cells were transfected with the indicated plasmids and treated 24 h later with 50 ng/ml TNF-α for 30 min. The cells were lysed and immunoblotted using the indicated antibodies. Tubulin and poly(ADP-ribose) polymerase (PARP) were used to normalize cytosolic and nuclear protein concentrations, respectively. The asterisks used in quantification panels indicate significantly different protein abundance as compared with the TNF-α control (n = 3, ANOVA). B, analysis of TRAF2 polyubiquitination. HEK 293T cells were transfected with the indicated plasmids and treated 24 h later with 50 ng/ml TNF-α for 5 min. Cell lysates were immunoprecipitated (IP) using FLAG antibody and immunoblotted for ubiquitin (Ub). Asterisks used in quantification panels indicate significantly different TRAF2-Ub signal intensity as compared with the TNF-α control (n = 3, ANOVA). C. rod, C. rodentium.