. 2017 May 18;292(27):11423–11430. doi: 10.1074/jbc.M117.790675

Table 1.

Primers used in this study

Description	Sequence
C. rodentium nleB BamHI forward	G₄ATC₂ATGT₂ATCTC₂AT₂A₃TGT₂C
C. rodentium nleB XhoI reverse	C₃TCGAGC₂ATGA₂CTGT₂G₂TATAC
C. rodentium nleB NleB forward	ATATCTAGAGC₂AC₂ATGTAC₃ATACGACGTC₃G₂ACTACGCGT₂ATCTC₂AT2A₃T
C. rodentium nleB NleB reverse	ATATGCG₂C2GCT₂AC₂ATGA₂CTGT₂G₂TATACAT
EHEC nleB1 NcoI forward	ATCGCATATGC₃ATG₂CAT₂ATCT₂CAT₂A₃TGTC₂T₂CA₂T
EHEC nleB1 SalI reverse	ATCG₂TCGAC₃ATGA₂CTGCAG₂TATACATACTG₂T
EHEC nleB2 XbaI forward	ATATCTAGAGC₂AC₂ATGTAC₃ATACGACGTC₃G₂ACTACGCGC₃ATG₂CACT₃CA
EHEC nleB2 NotI reverse	ATATGCG₂C₂GCT₂AC₂ATGA₂CTGCATGTATACTGACT₂
EHEC nleB2 BamHI forward	CGCG₃ATC₂ATGCT₃CAC₂GATA₂G₂ACA₂CT₃C
EHEC nleB2 XhoI reverse	CGCGCTCGAGC₂ATGA₂CTGCATGTATACTGACT
EHEC nleB1 XbaI forward	ATATCTAGAGC₂AC₂ATGTAC₃ATACGACGTC₃G₂ACTACGCGT₂ATCT₂CAT₂A₃T
EHEC nleB1 NotI reverse	ATATGCG₂C₂GCT₂AC₂ATGA₂CTGCAG₂TATACATACTG₂T
EPEC nleB1 NcoI forward	ATCGCATATGC₃ATG₂CAT₂ATCT₂CAT₂A₃TGTC₂T₂CA₂T
EPEC nleB1 SalI reverse	ATCG₂TCGAC₃ATGA₂CTGCTG₂TATACATACT
EPEC nleB1 NotI reverse	ATATGCG₂C₂GCT₂AC₂ATGA₂CTGCTG₂TATACATACT
FADD NdeI forward	CG₂A₂T₂CTATATG₃TGA₂GA₂GATCTGTGCGC₂
FADD BamHI reverse	CG₂A₂T₂CG₂ATC₂TCA₂GCGC₂GCTACGAT₄GCAG
GAPDH BamHI forward	G₄ATC₂ATG₄A₂G₂TG
GAPDH XhoI reverse	C₃TCGAGCTC₂T₂G₂AG₂
GAPDH R200A forward	G₂A₃CTGTG₂CGTGATG₂CGC₂G₄CTCTC₂
GAPDH R200A reverse	G₂AGAGC₃G₂CGC₂ATCACGC₂ACAGT₃C₂
GAPDH R197A forward	G₂A₃CTGTG₃CTGATG₂C₂GCG₃CTCTC₂AGA₂CATCATC₂
GAPDH R197A reverse	G₂ATGATGT₂CTG₂AGAGC₄GCG₂C₂ATCAGC₃ACAGT₃C₂
GAPDH R197A,R200A forward	G₃A₃CTGTG₃CTGATG₂CGCTG₄CT
GAPDH R197A,R200A reverse	AGC₄AGCGC₂ATCAGC₃ACAGT₃C₃
GAPDH R197K forward	G₂ATG₂C₄TC₂G₃A₃CTGTG₂A₂G₂ATG₂C₂GCG₄CTCT
GAPDH R197K reverse	AGAGC₄GCG₂C₂ATC₂T₂C₂ACAGT₃C₃G₂AG₄C₂ATC₂
GAPDH R200K forward	G₂ATG₂C₄TC₂G₃A₃CTGTG₂CGTGATG₂CA₂G₅CTCTC₂AGA₂CATC
GAPDH R200K reverse	GATGT₂CTG₂AGAGC₄CT₂GC₂ATCACGC₂ACAGT₃C₃G₂AG₄C₂ATC₂
GAPDH R197K,R200K forward	G₂ATG₂C₃TC₂G₃A₂CTGTG₂A₂G₂ATG₂CA₂G₅CTCTC₂AGA₂CATC
GAPDH R197K,R200K reverse	GATGT₂CTG₂AGAGC₄CT₂GC₂ATC₂T₂C₂ACAGT₃C₃G₂AG₄C₂ATC₂
GAPDH R197A,R200A forward	G₃A₃CTGTG₃CTGATG₂CGCTG₄CT
GAPDH R197A,R200A reverse	AGC₄AGCGC₂ATCAGC₃ACAGT₃C₃
GAPDH shRNA UTR-1 forward	GATC₂G₂AGC₂GCAC₂T₂GTCATGTACTCA₂GAG₂TACATGACA₂G₂TGCG₂CTCT₆G₂
GAPDH shRNA UTR-1 reverse	A₂T₂C₂A₆GAGC₂GCAC₂T₂GTCATGTAC₂TCT₂GAGTACATGACA₂G₂TGCG₂CTC₂G
Salmonella sseK1 XbaI forward	ATATCTAGAGC₂AC₂ATGTAC₃ATACGACGTC₃G₂ACTACGCGATC₃AC₂AT₂A₃T
Salmonella sseK1 NotI reverse	ATATGCG₂C₂GC₂TACTGCACATGC₂TCGC₃ATGA₂CT
Salmonella sseK2 XbaI forward	ATATCTAGAGC₂AC₂ATGTAC₃ATACGACGTC₃G₂ACTACGCG₂CACGT₄A₂TGC₂
Salmonella sseK2 NotI reverse	ATATGCG₂C₂GCT₂AC₂TC₂A₂GA₂CTG₂CAGT₂A₃CT
Salmonella sseK3 XbaI forward	ATATCTAGAGC₂AC₂ATGTAC₃ATACGACGTC₃G₂ACTACGCGT₄CTCGAGTCAGA
Salmonella sseK3 NotI reverse	ATATGCG₂C₂GCT₂ATCTC₂AG₂AGCTGATAGTCA₃
Salmonella sseK1 BamH1 forward	CGCG₃ATC₂ATGATC₃AC₂AT₂A₃TAGATATGT₂C
Salmonella sseK1 XhoI reverse	CGCGCTCGAGCTGCACATGC₂TCGC₃ATGA₂
Salmonella sseK2 BamHI forward	CGCG₃ATC₂ATG₂CACGT₄A₂TGC₂GCT₄A
Salmonella sseK2 XhoI reverse	CGCGCTCGAGC₂TC₂A₂GA₂CTG₂CAGT₂A₃CT
Salmonella sseK3 NcoI forward	CGCGC₂ATG₂T₃CTCGAGTCAGAG₂T₄CT₃C
Salmonella sseK3 SalI reverse	CGCG₂TCGACATC₂TCTC₂AG₂AGCTGATAGTC
TRAF2 BamHI forward	GCG₂ATC₂ATG₂CTGCAGCTAGCGTGAC₂
TRAF2 HindIII reverse	GCA₂GCT₂G₂AGC₃TGTCAG₂TC₂ACA₂TG₂