Figure 1.

Amino acid sequence of 15 different anti-EphA4 LBD Nbs. Nbs were numbered according to Kabat numbering with reference sequence VH3–23/JH5 (human germ line sequence on average most closely related to Nanobody sequences) on top. The complementary determining regions were assigned according to the AbM definitions (75) and are shown with a gray background. The Nbs belonged to nine clonally unrelated B-cell clones, and the different groups are shown alternatively in black and red. According to the amino acid sequences Nb 90 and 60, Nb 19 and 57, Nb 34 and 47, and Nb 31, 50, 10, and 53 belonged to the same group. Nb 39, 16, 71, 28, and 22 belonged to unrelated B-cell clones.