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. 2017 May 19;292(27):11452–11465. doi: 10.1074/jbc.M116.774141

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version free via Creative Commons CC-BY license.

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Figure 2. — Nbs bind native EphA4 LBD. Nbs and EphA4 co-immunoprecipitated (IP) together, both when immunoprecipitating different Nbs (left panel) or when immunoprecipitating EphA4 (right panel). A and B, Nb 53 and Nb 39 could capture EphA4 and could be captured by EphA4. C, Nb 22 showed lower affinity for mouse EphA4 compared with human EphA4. D and E, Nb 19 and 47 showed unspecific binding to the beads. F, Nb 60 showed cross-reactivity with ephrin-B2, which was performed as a negative control experiment.