Figure 5.

A metal-binding ectodomain is not sufficient to induce Ctr2-mediated rescue of respiration-dependent growth. A, model showing monomeric full-length human Ctr1 and Ctr2 along with a chimeric protein consisting of the first 42 residues of Ctr1 fused to Ctr2. Metal-binding Met and His residues are indicated in blue and red, respectively, and glycans are indicated with branches. B, the indicated proteins were expressed in S. cerevisiae ctr1Δctr3Δ cells and plated onto the indicated growth medium. C, the same cells as in B were evaluated by fluorescence microscopy and photographed. DIC, differential interference contrast.