Figure 8.

Ctr2 transport activating mutations impart selectivity for Cu⁺ and increase cadmium sensitivity. A, S. cerevisiae ctr1Δctr3Δ and ctr1Δctr3Δfre1Δ cells were transformed with the indicated expression plasmids and plated on the indicated medium, with wedges indicating decreasing cell density plated. B, S. cerevisiae ctr1Δctr3Δ cells were transformed with the indicated plasmids, transferred to medium containing the indicated concentration of AgNO₃, and grown for 24 h with agitation at 30 °C. Data are shown as percent growth of strain grown in medium without treatment, with the histogram displaying data for growth in 1.56 μm AgNO₃. C, S. cerevisiae ctr1Δctr3Δ cells were transformed with the listed plasmids and transferred to medium containing the indicated amount of CdCl₂ and grown for 24 h with agitation at 30 °C. Data are shown as percent growth of strain grown in medium without treatment, with the histogram displaying data for growth in 1.95 μm CdCl₂. *, p ≤ 0.05; ***, p ≤ 0.001.