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. 2017 Jul;187(7):1523–1536. doi: 10.1016/j.ajpath.2017.02.019

Figure 7.

Figure 7

Transient knockdown of L-plastin and vimentin inhibits invasion in vitro. A: Representative Western blots of L-plastin and vimentin and β-actin loading control and their quantification. B: Quantitative real-time RT-PCR (RT-qPCR) analysis of the mRNA abundance of L-plastin and vimentin in UM-SCC-1 siRNA transfections. C: Epidermal growth factor (EGF)–stimulated (0.1 nmol/L EGF) in vitro invasion was evaluated with representative examples of invasion filters provided underneath. ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.