Fig. 3. Ability of peptides to induce T cell activation. Activation of the NLV-specific clone was measured by intracellular staining for the cytokine TNFα and analysed by flow cytometry. Results are given as the percent TNFα positive cells of the NLV-specific clone cells. NLV refers to the peptide NLVPMVATV, which served as a positive control for the assay. APC were prepared from 3 individual donors as shown; each compound was tested in duplicate. Statistical significance for each sample (compared to 1) was calculated using a one way Anova followed by Dunnett's multiple comparison test; *p < 0.05, **p < 0.01, ***p < 0.001.