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. 2017 Jul 4;8:15857. doi: 10.1038/ncomms15857

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Strong apical cytoplasmic induction of Deup1 in Tm-treated E2f4^f/f;R26^CreERT2/+ transduced with E2F4^WT (arrow in top panel: ALI day6 cells); HA-Deup1-Pcm1 aggregates confirmed in side projection views at ALI day2 (circled area in bottom panel). (b) E2F4^WT-transduced cells express HA, E2f4, and Pcm1 in overlapping patterns in large and smaller cytoplasmic granules (single channels and double-labelled low magnification panels; inset: representative double-labelled cell at high magnification; DAPI). (c) E2F4^ΔDBD-transduced cells: HA expression in nucleus and cytoplasm but no Deup1 or Pcm1 signals. (d) E2F4^ΔNES-transduced cells: HA expression selectively nuclear; Deup1 mislocalized in nuclei (arrow) and unable to form apical aggregates with Pcm1. All bars=5 μm.