Figure 5. Expansion of Methyltransferase in P. cynomolgi and P. simiovale.

( A) Tree of methyltransferase in Plasmodium, including the expansion of those genes in P. cynomolgi (36) and P. simiovale (at least 15). The closest core genes are PVP01_0943400 and PcyM_0947500. ( B) Comparative view of P. cynomolgi and P. vivax on the locus of methyltransferase (*) of panel A. Interestingly, the locus in P. cynomolgi has an insertion with a subtelomeric gene that has a weak hit with to a putative DNA translocase Ftsk domain. Coverage plot mapped from P. cynomolgi reads (black), P. vivax (blue) and P. simiovale (magneta) is shown in log scale on P. cynomolgi. The methyltransferases are duplicated more than 35 times. As the height is roughly similar between the two duplications, we expect around the same number of methyltransferases in P. simiovale than in P. cynomolgi. The insert of the green gene is found just in P. cynomolgi, due to the missing coverage. The upper panel shows the distance of read pairs; the insertion of the region probably occurred after the duplication of the gene into the subtelomeres, as all reads from the duplications are connected over the insertion. The next core gene is also duplicated.